April 20, 2017

Download Animal Cell Technology: Basic & Applied Aspects: Proceedings by Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji PDF

By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)

Animal phone know-how is a becoming self-discipline of phone biology which goals not just to appreciate buildings, features and behaviors of differentiated animal cells, but in addition to examine their skills for use for commercial and scientific reasons. The objective of animal phone expertise comprises the clonal growth of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of scientific and pharmaceutical importantance, and the appliance of animal cells to gene remedy, man made organs and the creation of sensible meals. This quantity provides the readers an entire evaluation of the current cutting-edge and may be necessary for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, rather phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines relating to animal telephone culture.

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Read or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF

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Extra resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006

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Immediately after electrophoresis, the gels were fixed overnight in 50% methanol and 10% acetic acid. The gels were then stained using Ez Stain Silver (ATTO, Japan). 1 Examination for Determining Optimal Concentration of Sericin To determine optimal concentration of sericin, hybridoma cells were cultured in the presence of sericin at various concentrations. As shown in Fig. 1, the cell proliferation rates in the presence of sericin were higher than those in its absence. 15%. 1%. 2 Proteome Analysis Using 2-DE To investigate the protein profiles of 2E3-O cells treated with sericin, 2-DE was performed.

IL-2 signaling also induces activation of phosphatidylinositol 3 kinase (PI3K) and its downstream effector Akt, and the tyrosine phosphorylation of IL-2Rb results in the recruitment of Shc and Grb2, followed by activation of the Ras/ MAPK pathway. The main functional outcomes of these signaling cascades are cell proliferation and cytoskeletal regulation. To examine whether the chimeric IL-2Rs mimic IL-2 signaling or not, phosphorylations of STAT5, STAT3, and ERK1/2 were detected by Western blotting (Fig.

1 Effect of cytokines on 2E3 cell proliferation. 1–10 ng/ml (b). 2E3 cells were cultured in serum-free ASF104 medium 50 M. Tanaka et al. 0 10 LIF concentration (ng/ml) Fig. 2 Effect of cytokines on proliferation of 2E3-O cells adapted to serum-free medium. 1–10 ng/ml (b). 0 10 LIF concentration (ng/ml) d c 1 60 Viable cell density at day 3 (104 cells /ml) 50 2 3 4 5 bp 50 40 1000 30 500 20 10 0 200 - IL-6 TGF-β Cytokine Fig. 3 Effect of cytokines on viability of 2E3-O cells adapted to serum-free medium: (a) IL-6, (b) LIF, (c) inhibition of the growth of 2E3-O cells in the presence of cytokines at 5 ng/ml, (d) gel electrophoresis for detection of DNA fragmentation.

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