By Margaret I. Tyler (auth.), Catherine Cooper, Nicolle Packer, Keith Williams (eds.)
Amino acid research is typical in biotechnology, biomedical, and meals research laboratories. Amino Acid research Protocols constitutes a massive selection of those integral analytical concepts, either vintage and state-of-the-art, of excessive application for answering particular organic questions. universal tools contain these according to HPLC or gasoline chromatography separation and research after precolumn derivatization. New recommendations in response to capillary electrophoresis separation, high-performance anion alternate chromatography, and mass spectrometry also are provided. for the reason that effects rely seriously at the caliber of the pattern, such a lot individuals have committed a bit to pattern instruction, really to the gathering and garage of physically fluids. a brand new technique for desalting samples ahead of hydrolysis is usually supplied. each one approach is defined in step by step element to make sure profitable experimental effects, and comprises worthwhile notes on pitfalls to prevent, and diversifications that let the how you can be used with varied systems.
up to date and hugely useful, Amino Acid research Protocols bargains analytical and scientific chemists, in addition to a vast variety of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the efficient research of either universal and unusual amino acids.
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Extra info for Amino Acid Analysis Protocols
5. Cohen, S. A. and Strydom, D. J. (1988) Amino acid analysis utilizing phenylisothiocyanate derivatives. Anal. Biochem. 174, 1–16. 6. Cohen, S. A. and Michaud, D. P. (1993) Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high performance liquid chromatography. Anal. Biochem. 211, 279–287. 7. Liu, H. J. (1994) Determination of amino acids by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and high performance liquid chromatography with ultraviolet detection.
A. (1993) Sensitive analysis of cystine/cysteine using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatives, in Techniques in Protein Chemistry IV (Angeletti, R. H. ), Academic, San Diego, CA, pp. 299– 306. 10. Cohen, S. A. and De Antonis, K. M. (1994) Applications of amino acid analysis derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Analysis of feed grains, intravenous solutions and glycoproteins. J. Chromatog. 661, 25–34. 11. Van Wandelen, C. and Cohen, S. A. (1997) Using quaternary high-performance liquid chromatography eluent systems for separating 6-aminoquinolyl-N- Precolumn Derivatization with AQC 47 hydroxysuccinimidyl carbamate-derivatized amino acid mixtures.
14. Samples or washes larger than 400 μL are accommodated by loading multiple aliquots into the reservoir. The PVDF membrane should not be allowed to dry between aliquots. 15. Based on a recent ABRF study on amino acid analysis (8), samples bound to PVDF membranes tend to have higher error rates (20%) than those analyzed free in solution (12%). These error rates are averages for the study. References 1. Dupont, D. , Keim, P. , Chui, A. , and Wilson, K. J. (1989) A comprehensive approach to amino acid analysis, in Techniques in Protein Chemistry (Hugli, T.
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